Chemical Constituents Analysis of Ethyl Acetate Extract from MSR-1707 by GC-MS

Aims: To analyze the chemical constituents of ethyl acetate extract from MSR-1707 to promote the rational utilization of the mushroom resources.

Methodology: MSR-1707 belongs to the genus Nigrospora sp. It was extracted by ethyl acetate, then the extract was analyzed by Gas Chromatography-Mass Spectrometer (GC-MS). Identification of compounds was achieved according to their GC retention indices (RI) and database search using the library of NIST05, as well as a comparison of the fragmentation pattern of the mass spectra with data published in the literature.

Results: Seventy-three compounds were separated by gas chromatography. Based on the NIST05 spectral library and corresponding literature information, fifty-three compounds were identified. Their relative percentage of contents accounted for 95.62% of the outflow peak. Some of the identified peaks are 9-Octadecenoic acid, methyl ester(E)(18.50%),9-Tricosene(Z)(8.30%), 13-Docosenamide (E)(5.26%) and Myristic acid glycidyl ester (3.11%).

Conclusion: This is the first report of chemical constituents of the ethyl acetate extract of Nigrospora sp. using GC-MS, which offer some theoretical basis for the further exploration and application of this mushroom.