Microdoses Levels of Butadiene Diepoxide (BDO2) Induced Toxicity in Prostate Cancer Cells

Koppula, Sowmya and Tan, Mei-Chyi and Hurst, Angela and Telles, Caroline and Gray, Wesley (2013) Microdoses Levels of Butadiene Diepoxide (BDO2) Induced Toxicity in Prostate Cancer Cells. Pharmacology & Pharmacy, 04 (02). pp. 209-217. ISSN 2157-9423

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Abstract

Low-level exposure to environmental pollutants such as BDO2 contributes directly and indirectly to an increase in PCa. The aim of this study was to define the cellular changes associated with micro-doses of Butadiene Diepoxide (BDO2) in prostate cancer cells. We observed that micro-doses of BDO2 resulted in dose- and time-dependent increases in cytotoxicity and increased expression of prostate tumor markers in LNCaP(AR+) and DU145(AR-) cells. There was an increased sensitivity of DU145(AR-) cells to BDO2 toxicity which was reversed by transient transfection of AR into theses cell. Exposure of prostate cells to BDO2 increases cytotoxicity, and apoptosis, which correlates with increases in caspases and Bcl2 protein and mRNA levels. In cell DU145(AR-) cell transient transfected with a functional AR, the levels of cytotoxicity and caspase activity were decreased in the presence of BDO2, but BDO2-induced apoptotic protein expression was unaltered. This study provides evidence that micro-doses of BDO2 modulate prostate cell toxicity by promoting apoptosis and tumor gene expression.

Item Type: Article
Subjects: East India Archive > Chemical Science
Depositing User: Unnamed user with email support@eastindiaarchive.com
Date Deposited: 01 Mar 2023 07:31
Last Modified: 28 Aug 2024 14:11
URI: http://ebooks.keeplibrary.com/id/eprint/397

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