Serodiagnosis of Hepatitis B Virus Using PreS Antigen from Pakistani Isolate SBS001

Iftikhar, Saima and Aslam, Farheen and Akhtar, Muhammad (2021) Serodiagnosis of Hepatitis B Virus Using PreS Antigen from Pakistani Isolate SBS001. Journal of Pharmaceutical Research International, 33 (42A). pp. 246-255. ISSN 2456-9119

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Abstract

Background: An estimated 325 million people worldwide live with hepatitis B and/or C and approximately, 5 million people are affected with hepatitis B. in Pakistan.This study aimed at developing PreS protein from Hepatitis B Virus Pakistani isolate (SBS001) with enhanced sensitivity to detect antibodies in serum as a diagnostic method.

Methods: Gene encoding PreS region from hepatitis B Virus was cloned and expressed in Escherchia coli. The recombinant protein preS-His was purified by Ni-IDA affinity chromatography. Antibodies were raised in rabbit. This protein was screened for detection of antibodies in HBV patients’ sera through ELISA. This ELISA procedure was compared with commercially available Kit used for diagnosis of HBV infection.

Results: Single band purified recombinant PreS protein was obtained with high titer of antibodies raised in rabbits. This recombinant protein was used in ELISA as antigen coated on the plate. That efficiently detected antibodies present in HBV patients. It was concluded that preS-His antigen/ protein A HRP-conjugate ELISA method was more sensitive than the commercial kit for detecting the antibodies present in HBV patient sera.

Conclusion: It was concluded that SBS001 PreS recombinant protein can be used in ELISA kits for detection of HBV in Pakistani population.

Item Type: Article
Subjects: East India Archive > Medical Science
Depositing User: Unnamed user with email support@eastindiaarchive.com
Date Deposited: 24 Mar 2023 09:44
Last Modified: 20 Sep 2024 04:27
URI: http://ebooks.keeplibrary.com/id/eprint/563

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