Effects of Berberine on Bloog Glucose, Glycated Hemoglobin A1, Serum Insulin, C–Peptide, Insulin Resistance and β– Cell Physiology

Objective: Determine effects of berberine (BBR) on bloog glucose, glycated hemoglobin A1, serum insulin, C – peptide, insulin resistance and β– cell physiology.

Study Design: Experimental study

Place and Duration: Department of Physiology Isra University and Animal house of Sindh Agriculture University, Tando Jam from August 2019 to February 2020.

Methodology: A sample of 100 adult Wistar male rats was selected according to criteria of study and divided into 5 groups through random technique. Group A – negative controls (N/S 0.9%), Group B – positive control (diabetic rats – Alloxan 120 mg/kg) (i.p), Group C – E; Diabetic rat + BBR (50, 100 and 200 mg/kg) respectively. Blood samples were collected from retro-orbital venous plexus by capillary tube. Sera were separated at 3000 rpm (15 minutes). Insulin resistance (HOMA-IR) and β – cell secretory activity (HOMA-β) were calculated by mathematical formula. Statistical analysis was performed on SPSS version 21.0 (IBM, incorporation, USA) at 95% CI (P≤ 0.05).

Results: The present study noted significant improvement of fasting and random blood glucose, glycated HbA1, serum insulin and C –peptide, insulin resistance (HOMA-IR) and β – cell physiology.

Conclusion: Berberine (BBR) ameliorates the bloog glucose, glycated hemoglobin A1, serum insulin, c – peptide, insulin resistance and β– cell physiology.