Anti-cytoskeleton Immunoscreening of Trypanosoma brucei Expression Library Reveals Novel Immunogenic Conserved Putative Proteins

Magyezi, Begumisa Godfrey and Uthman, Okalang and Kenneth, Musisi and Mwijjwiga, Wampadde Eddie and George, Lubega W. (2019) Anti-cytoskeleton Immunoscreening of Trypanosoma brucei Expression Library Reveals Novel Immunogenic Conserved Putative Proteins. Journal of Advances in Biology & Biotechnology, 22 (1). pp. 1-11. ISSN 2394-1081

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Abstract

Background: The overall shape of the trypanosome is defined by an internal cytoskeleton consisting of a network of microtubules that are cross linked both to each other and the inner face of the plasma membrane. However, the total compliment and identity of the trypanosome cytoskeleton proteins are not yet fully determined despite the fact that some of them may be good targets for diagnostics, drugs and/or vaccines discovery.

Methods: In this study, rabbit anti-Trypanosoma brucei detergent insoluble cytoskeleton sera were produced in vivo and used to probe a T. brucei expression library. The picked plaques were made clonal by a series of library screening followed by PCR amplification, cDNA sequencing and identification of the proteins coded by these sequences using BLAST.

Results: The previously well-known cytoskeleton proteins (paraflagella rod protein and histone H2B), putative cytoskeleton proteins (Dynein light chain and nucleoporin), conserved hypothetical protein (Tb10.61.2430) and novel cytoskeleton protein coding cDNA sequences (not in the sequenced and published T. brucei genome) were identified in this study.

Conclusion: This approach is therefore, useable in the search for novel proteins whose utility in the design and development of diagnostics, drugs and/or vaccines can further be studied.

Item Type: Article
Subjects: East India Archive > Biological Science
Depositing User: Unnamed user with email support@eastindiaarchive.com
Date Deposited: 08 Apr 2023 07:59
Last Modified: 23 Sep 2024 04:40
URI: http://ebooks.keeplibrary.com/id/eprint/676

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